Tests that differentiate pectolytic bacteria
From Bugwoodwiki
Purpose
Ingredients
| Test | Pcc | Pca | Pcb | Pco | Pcw | Dch | Pct |
|---|---|---|---|---|---|---|---|
| Growth at 37 °C | + | - | + | + | - | + | + |
| Reducing sugars from sucrose | - | + | + | + | - | - | - |
| Phosphatase activity | -b | - | - | - | - | + | V |
| Sensitivity to erythromycin | - | - | - | ND | - | + | - |
| Indole production | - | - | - | - | - | + | - |
| Acid produced from: | |||||||
| sorbitol | - | - | - | + | ND | - | - |
| Melibiose | + | + | - | + | - | + | - |
| citrate | + | + | - | + | + | ND | |
| Raffinose | + | + | - | ND | - | + | - |
| Arabitol | - | - | - | + | ND | - | ND |
| Lactose | + | + | + | + | + | + | - |
| Utilization of Keto-methyl glucoside | - | + | + | + | - | - | - |
Instructions
- +, 80% or more strains positive
- V, between 21-79% of strains positive
- -, 80% or more strains negative
- ND, not determined.
- a Pcc = P. carotovorum subsp. carotovorum
- Pca = P. carotovorum subsp. atroseptica
- Pcb = P. carotovorum subsp. betavasculorum
- Pco = P. carotovorum subsp. odorifera
- Pcw = P. carotovorum subsp. wasabiae
- Dch = Dickeya. chrysanthemi
- Pct = P. cacticida
- b Some strains of P. carotovorum subsp. carotovorum may be weakly positive
Notes
References
- Schaad , Jones and Chun. 2001. Laboratory guide for the identification of plant pathogenic bacteria, APS press, 373 pp.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
