View source for Acid potato dextrose agar (half strength)

==Purpose==
This medium is used specifically for culturing Colletotrichum cerealis from turfgrass.

==Ingredients==
{|class='wikitable'
!Ingredient
!align="right"|1 L
!align="right"|500 ml
|-
!Colspan='3'|Part 1
|-
|Distilled water
|align="right"|1000 ml
|align="right"|500 ml
|-
|Dehydrated Potato Dextrose Agar
|align="right"|19.5 g
|align="right"|9.75 g
|-
|Agar
|align="right"|7.5 g
|align="right"|3.75 g
|-
!Colspan='3'|Part 2
|-
|Lactic Acid
|align="right"|1 ml
|align="right"|.50 ml
|}

==Instructions==
#Mix distilled water, Potato dextrose agar and agar.
#Autoclave and cool to 50-55°C
#Concentrated lactic acid while stirring. 

==Notes==
*This medium reduces vegetative growth and can facilitate the culture and identification of many fungi. Some people use quarter strength APDA; adjust the agar accordingly to 1.5% total.

==References==

==Contributed by==
From the Culture Media for Plant Pathogenic Fungi and Bacteria; University of Massachusetts: contributed by [[user:Rwick|Robert L. Wick]]

[[Category:Fungal Non-selective Media]]