Purpose
This medium is useful in identifying Xanthomonas species. Xanthomonas colonies are mucoid, convex, and shiny on YDC agar
Ingredients
| Ingredient | 1 L | 500 mL |
|---|---|---|
| Distilled water | 1 L | 500 mL |
| Dextrose | 20 g | 10 g |
| Yeast extract | 10 g | 5 g |
| CaCO3, light powder | 20 g | 10 g |
| Agar | 20 g | 10 g |
Instructions
- Autoclave dextrose separately in half of the distilled water
- Autoclave other ingredients in the other half of the distilled water
- Combine solutions. Stir before pouring plates so that CaCO3 is evenly distributed.
Notes
- It is important to try to keep the calcium carbonate suspended in the medium
References
- Schaad, N. W. 1988. Laboratory Guide for the Identification of Plant Pathogenic Bacteria. The American Phytopathological Society, St. Paul, MN. pp. 81-82.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen
