Xanthomonas selective medium
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Purpose
For culturing Xanthomonas.
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Part 1 | ||
| K2HPO4 | 3 g | 1.5 g |
| NaH2PO4 | 1 g | 0.5 g |
| NH4cl | 1 g | 0.5 g |
| MgSO4 • 7H2O | 0.3 g | 0.15 g |
| Bacto agar | 15 g | 7.5 g |
| Part 2 | ||
| Cellobiose | 10 g | 5 g |
| Cyclohexamide | 0.2 g | 0.1 g |
| L-Glutamic acid | 0.005 g | 0.0025 g |
| L-Methionine | 0.001 g | 0.001 g |
| Cephalexin | 0.01 g | 0.005 g |
| Bacitracin | 0.01 g | 0.005 g |
Instructions
- Autoclave the ingredients from part 1
- After medium is cooled to 60 °C, add ingredients from part 2 that have been filter-sterilized
Notes
- Plates should be without surface moisture prior to use.
References
- Kado, C.I. and Heskett, M.G. 1970. Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology 60: 969-976.
- Cubeta, M.S. and T.L. Kuan. 1986 Comparison of MD5 and XCS media and development of MD5A medium for detecting Xanthomonas campestris pv. carotae in carrot seed. (Abstr.) Phytopathology 76: 1109.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
