V8 agar
From Bugwoodwiki
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This protocol has been reviewed by the National Plant Diagnostic Network Diagnostics Committee. |
Purpose
Excellent for the sporulation of various fungi, but especially for Thielaviopsis basicola.
Ingredients
| Ingredient | 1 L | 500 mL |
|---|---|---|
| distilled water | 800 mL | 400 mL |
| V8 juice | 200 mL | 100 mL |
| CaCO3 | 2 g | 1 g |
| agar | 15 g | 7.5 g |
Instructions
- Mix ingredients
- Autoclave and cool to 50-55°C
Notes
- Recipes for this medium vary with respect to the amount of V8 juice added. Some use one 6 oz. can (180 ml/liter) because it is convenient.
- The calcium carbonate is reported to prevent the acidity of the juice from hydrolyzing the agar.
- Rob Wick reports no problems with solidification when CaCO3 was lacking.
- This medium is often used to grow Pythium and Phytophthora.
- If it is important to view microscopic features directly on the medium, it should be clarified by filtration or centrifugation before sterilization.
References
- Dhingra, O. D., and J. B. Sinclair. 1985. Basic Plant Pathology Methods. CRC Press, Inc., Boca Raton, FL. 355 pp.
- Stevens, R. B. 1974. Mycology Guidebook. University of Washington Press, Seattle, WA. 703 pp.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Originally created by Robert Wick; contributed by Mary Ann Hansen
