Tetrazolium medium
From Bugwoodwiki
Purpose
Used to test for pathogenicity of the vascular bacterial pathogen, Ralstonia solanacearum
Materials
| Ingredient | 500 mL | 1 L |
|---|---|---|
| distilled water | 500 mL | 1 L |
| peptone | 5 g | 10 g |
| casein hydrolysate | 0.5 g | 1 g |
| glucose | 2.5 g | 5 g |
| agar | 8.5 g | 17 g |
| triphenyl tetrazolium chloride | 0.025 g | 0.05 g |
Instructions
- Mix
- Autoclave
- Pour and cool
Expected results
- Two colony types appear on this medium. The nonpathogenic or weakly pathogenic colonies are round butyrous, deep red with narrow bluish borders. The highly pathogenic colonies are irregularly round, fluidal, white colonies or white colonies with pink centers. Clavibacter will not grow on this medium.
Notes
Images
 |
References
- Kelman, A. 1954. The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44: 693 695.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen
