TAB for Rhizoctonia
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Purpose
Ingredients
| Ingredient | 1 L | 3 L | 8 L |
|---|---|---|---|
| Part A | |||
| Agar (Morehead) | 15 g | 45 g | 120 g |
| MgSO4 | 1.02 g | 3.06 g | 8.16 g |
| KH2PO4 | 1 g | 3 g | 8 g |
| CuSO4 20 mg (5H2O = 31.29mg) | 0.031 g | 0.094 g | 0.25 g |
| H2O | 800 ml | 2400 ml | 6400 ml |
| Part B | |||
| Casein hydolysate | 0.8 g | 2.4 g | 6.4 g |
| Glycerol | 2 ml | 6 ml | 16 ml |
| Tannic acid | 0.12 g | 0.36 g | 0.96 g |
| H2O | 200 ml | 600 ml | 1600 ml |
| Fungicides & Antibiotic | |||
| Neomycin sulfate (in water blank) | 0.7 g | 2.10 g | 5.6 g |
| Apron 25 (95 mg AI=380 mg Apron 25) | 0.38 g | 1.14 g | 1.52 g |
| Benlate 50 WP (2mg AI=4MG 50mg WP) | 0.004 g | 0.012 g | 0.032 g |
Instructions
- Make two parts (A&B)
- Autoclave seperately, cool to 50°C.
- Mix A&B (add 200 ml B to each 800 ml A), adjust pH to 6.5 with 1 NaOH.
- Add fungicides & antibiotic.
Notes
- Measure A&B with sterile graduated cylinder)
References
Contributed by
Contributed by Sherrie Smith, the University of Arkansas.
