Sucrose peptone agar
From Bugwoodwiki
Purpose
This medium is a nonselective medium that is useful for general purpose bacterial isolations; however, it is not recommended for culturing Agrobacterium. It is particularly useful when looking for levan-producing pseudomonads. Xanthomonas campestris is characteristically mucoid on this medium. The clarity of this medium makes it useful for plate counts.
Ingredients
| Ingredient | 1 L | 500 mL |
|---|---|---|
| distilled water | 1 L | 500 mL |
| sucrose | 20 g | 10 g |
| peptone | 5 g | 2.5 g |
| K2HPO4 | 0.5 g | 0.25 g |
| MgSO4•7H2O | 0.25 g | 0.125 g |
| agar | 15 g | 7.5 g |
Instructions
- Combine ingredients
- Adjust pH to 7.2-7.4 with 40% NaOH
- Mix and autoclave
Notes
References
- Fahy, P. C., and G. J. Persley. 1983. Plant Bacterial Diseases, A Diagnostic Guide. Academic Press, New York. 393 pp.
- Hayward, A. C. 1960. A method for characterizing Pseudomonas solanacearum. Nature 186: 405-406.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert L. Wick; contributed by Mary Ann Hansen
