A standard format for diagnostic media recipes can be very useful for comparing variations and switching between various resources. This article provides some basic guidelines and a standard format for recipes in the Diagnostician's cookbook. I highly recommend copying over the example listed at the bottom when you start a new recipe. It will most likely be easier than starting from scratch.

Basic guidelines

All measurements should be in metric

Starting template

This is the standard template for recipes in the Diagnostician's Cookbook. To use it, Highlight the contents of the shaded box, copy it, and paste it in the article for the new recipe.

==Purpose==
What does the media do that is special

==Ingredients==
{|class='wikitable'
!Ingredient
!align="right"|*Replace with total end volume*
!align="right"|*Replace with 2nd total end volume*
|-
|Ingredient1
|align="right"|Amount needed1
|align="right"|Amount needed2
|-
|Ingredient2
|align="right"|Amount needed1
|align="right"|Amount needed2
|-
|Ingredient3
|align="right"|Amount needed1
|align="right"|Amount needed2
|-
|Ingredient4
|align="right"|Amount needed1
|align="right"|Amount needed2
|-
|Ingredient5
|align="right"|Amount needed1
|align="right"|Amount needed2
|}

==Instructions==
#First Step
#Second Step
#Third Step
#Fourth Step

==Notes==
Special use of handling notes or other considerations

==References==
*Any original references for the recipe. The idea for this template was originally created by Robert Wick, Virgina Tech
*Next Reference

==Contributed by==
The name of the person contributing the recipe in case there are questions (preferably linked to their user account 
(i.e. [[User:NAME|Display Name]].

[[Category:What ever type of media it is]]

(You can have as many categories as needed. For a list of current categories, see the Disgnostician's Cookbook Discussion page)

This wiki Template contributed by Joe LaForest. He will be glad to help you edit it to meet your needs.

Example: High sucrose medium

Purpose

Diagnostic for Erwinia amylovora

Ingredients

Ingredient	1 L	500 mL
distilled water	760 mL	380 mL
sucrose	320 g	160 g
nutrient agar	24 g	12 g
0.1% crystal violet in absolute alcohol	1.6 mL	0.8 mL
0.1% cycloheximide	40 mL	20 mL

Instructions

Mix water and sucrose first, bringing it to a boil to dissolve, then add all other ingredients (except 0.1% cycloheximide) until they dissolve.
Autoclave and cool
Add 0.1% cycloheximide
pour plates and allow to dry for 2 hours prior to use or storage
Incubate cultures at 28°C for 60 hours. Examine for cratering of colony surface.

Notes

Erwinia amylovora colonies will develop "craters" on the colony surface. Craters can be seen as soon as colonies are large enough for viewing, usually in 48 72 hr, but are most distinct after 60 hr. Colony surface should be viewed on a stereoscope using oblique light. Erwinia herbicola and some other saprophytic isolates can grow on this medium, but do not show cratering.

References

Crosse, J. E. and R. N. Goodman. 1973. A selective medium for a definitive colony characteristic of Erwinia amylovora. Phytopathology 63: 1425 1426.

Contributed by

From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen

Standard format for diagnosticians recipes

Contents