Soil extract
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Purpose
For inducing sporangium formation in water molds.
Ingredients
- 500 ml dH2O
- 3 heaping teaspoons soil (any good garden soil)
Instructions
- Mix and place in autoclave.
- Autoclave for 20 m.
- About 24 hours later, autoclave again for 20 m.
- Place chunks of agar from nonselective medium containing mycelium of Pythium or Phytophthora in soil extract for 24-48 hours under light to induce sporangium formation.
Notes
References
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Originally created by Robert Wick; contributed by Mary Ann Hansen
