Rye medium A (modified)
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Purpose
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Rye broth | 1000 ml | 500 ml |
| Glucose | 10 g | 5 g |
| Agar | 15 g | 7.5 g |
Instructions
- Spread rye seed out on a piece of white paper and pick out ergot sclerotia and other contaminants.
- Weigh out 100 grams of cleaned seed and place in a sieve and rinse under running water, drain.
- Place the rinsed rye seed into 500 cc of distilled water, bring to a boil and turn off the heat. Allow to steep for 30 minutes.
- Strain steeped rye seed through several layers of cheese cloth into a 1 liter graduated cylinder; bring the volume up to one liter with distilled water.
- Autoclave and cool to 50 °C, then add the ingredients (the powders can be suspended in a 10 ml sterile water blank):
Notes
References
- Erwin, D. C. and Ribeiro, O. K. 1996. Phytophthora diseases worldwide. APS Press, St. Paul MN. 562 pp.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
