Nutrient agar
From Bugwoodwiki
Purpose
A good all purpose isolation and maintenance medium. Use the nutrient broth for shake culture.
Ingredients
| Ingredient | 1 L | 500 mL |
|---|---|---|
| Distilled water | 1 L | 500 mL |
| beef extract | 1 g | 0.5 g |
| Yeast extract | 2 g | 1 g |
| Peptone | 5 g | 2.5 g |
| Sodium chloride (NaCl) | 5 g | 2.5 g |
| agar | 15 g | 7.5 g |
Instructions
- Mix and autoclave
Notes
- A good all-purpose isolation and maintenance medium.
- This medium should be used for Dickeya rather than Kings medium B. Dickeya will die if cultured on KB. Use the broth for shake-culture.
References
- Fahy, P. C., and G. J. Persley. 1983. Plant Bacterial Diseases, A Diagnostic Guide. Academic Press, N. Y., NY. 393 pp.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen
