Minimal medium (Fusarium)
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For other uses, see Minimal medium (disambiguation).
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Purpose
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Distilled water | 1000 ml | 500 ml |
| Sucrose | 30 g | 15 g |
| MgSO4 • 7H2O | 0.5 g | 0.25 g |
| KC1 | 0.5 g | 0.25 g |
| FeSO4 • 7H2O | 10 mg | 5 mg |
| NaNO3 | 2 g | 1 g |
| Trace elements* | .2 ml | .1 ml |
| Agar | 20 g | 10 g |
Trace elements (stock solution)
| Ingredient | 100 ml |
|---|---|
| Distilled water | 95 ml |
| Citric acid | 5 g |
| Zn SO4 • 7H2O | 5 g |
| Fe(NH4)2(SO4) • 6H2O | 1 g |
| CuSO4 • 5H2O | 0.25 g |
| MnSO4 • H2O | 0.05 g |
| H3BO4 (boric acid) | 0.05 g |
| NaMoO4 • 2H2O | 0.05 g |
Instructions
- Autoclave for 20 minutes.
Notes
References
Reference: Correll, et al.. 1987 Phytopath 77:1640-1646.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
