Mefenoxam medium

From Bugwoodwiki

Purpose

Ingredients

Ingredient 1 L 500 ml
Distilled water 1000 ml 500 ml
Corn meal agar 17 g 8.5 g

Instructions

  1. Add mefenoxam aseptically with pipette to make the following concentrations:

Mefenoxam (Ridomil) 47.6%

100 ppm ...........................................................................210.0 μl ..........105.0 μl

50 ppm .............................................................................105.0 μl ............52.5 μl

25 ppm ...............................................................................52.5 μl ............27.0 μl

or

(Mefenoxam (Subdue) 21.3%)

(100 ppm)....................................................................... (470.0 μl)....... (235.0 μl)

(50 ppm)......................................................................... (235.0 μl)....... (117.5 μl)

(25 ppm)......................................................................... (117.5 μl)......... (59.0 μl)

 2. Autoclave for 20 minutes.

Notes

  • Mefenoxam is viscous and difficult to pipette; pipette slowly.

References

Contributed by

From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick