Malt salt agar
From Bugwoodwiki
Purpose
This medium is used to culture xerotrophic seed colonizing fungi such as Aspergillus flavus parasiticus group. The osmotic potential of the medium is high, due to the concentration of salt. This mimics the osmotic potential of seeds and preferentially selects for Aspergillus.
Ingredients
| Ingredient | 500 mL | 1L |
|---|---|---|
| distilled water | 500 mL | 1 L |
| Malt extract | 10 g | 20 g |
| Agar | 7.5 g | 15 g |
| NaCl | 37.5 g | 75 g |
Instructions
- Mix
- Autoclave
- Pour plates and allow to cool
Notes
- Do not autoclave more than 15 minutes.
- 40 mg/L of chlorotetracycline hydrochloride can be added to cooled 50°C medium to inhibit bacteria.
References
- Personal communication, Gary Griffin, VPI & SU, Blacksburg, VA.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen.
