MMC medium
From Bugwoodwiki
 |
There is a question about this article that needs to be resolved. Please see the discussion tab of this article for further explanation. |
|
Purpose
for non-nitrogen utilizing colonies (NITs)
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Distilled water | 1000 ml | 500 ml |
| Sucrose | 30 g | 15 g |
| KH2PO4 | 1 g | 0.5 g |
| MgSO4 • 7H2O | 0.5 g | 0.25 g |
| KCl | 0.5 g | 0.25 g |
| FeSO4 • 7H2O | 0.01 g | 0.005 g |
| Agar | 20 g | 10 g |
| Trace element solution* | 0.2 ml | 0.1 ml |
| L-Asparagine | 1.6 g | 0.8 g |
| NaNO3 | 2 g | 1 g |
| KClO3 | 15 g | 7.5 g |
Trace element solution*
| Ingredient | 100 ml |
|---|---|
| Distilled water | 95 ml |
| Citric acid | 5 g |
| ZnSO4 • 7H2O | 5 g |
| FeSO4 • 7H2O | 4.75 g |
| Fe(NH4)2(SO4)2 • 6H2O | 1 g |
| CuSO4 • 5H2O | 250 mg |
| MnSO4 • H2O | 50 mg |
| H3BO3 | 50 mg |
| Na2MoO4 • 2H2O | 50 mg |
Instructions
Notes
References
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
