Lactose broth
From Bugwoodwiki
Purpose
Used for detection of the presence of coliform organisms, as a pre-enrichment.
Ingredients
| Ingredient | 500 ml | *250 ml |
|---|---|---|
| Distilled water | 500 ml | 250 ml |
| Bacto beef extract | 1.5 g | 0.75 g |
| Bacto peptone | 2.5 g | 1.25 g |
| Bacto lactose | 2.5 g | 1.25 g |
Instructions
- Dissolve ingredients and dispense medium into test tubes containing an inverted Durham tube.
- Autoclave (air bubbles are expelled from the Durham tube during autoclaving).
- Inoculate tubes and place in a water bath at 35 or 44.5 °C.
- Observe at 24 and 48 hours for an air bubble in the Durham tube.
Notes
- The coliform group is Enterobacteriaceae that ferment lactose with gas formation within 48 hours at 35 °C. Fecal coliforms are detected by lactose fermentation on M-FC Broth (see standard methods).
References
- Difco Manual, 10th Ed. 1984. Difco Laboratories, Detroit, MI. 1155 pp.
- American Public Health Association. 1985. Standard methods for the examination of water and wastewater.
Contributed by
The name of the person contributing the recipe in case there are questions (preferably linked to their user account (i.e. Display Name.
