L-asparagine solution
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Purpose
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Autoclaved dH2O | 950 ml | 470 ml |
| L-asparagine (in a 10 ml water blank) | 2 g | 1 g |
Instructions
- All glassware must be acid-soaked, then thoroughly rinsed in 4 changes of distilled water, and dried before use.
- Prepare the 4 solutions.
- Filter-sterilize the L asparagine solution into the autoclaved water.
- Then filter-sterilize, in the following order: solutions #3,#4,#2,#1, at a concentration of 10 ml/liter (5 ml/500 ml) into the sterile asparagine solution.
- Dispense in 5 ml quantities in test tubes.
- Inoculate from weak suspensions. Include a positive growth control (a non-Xanthomonas species).
- Examine tubes for growth after 4, 7, and 10 days of shaker incubation. Weak growth is checked by transferring a 2 mm loopful of the 10 day old culture to fresh tubes of the asparagine medium and to YS broth. Repeat if weak growth occurs.
Notes
References
- Dye, D. W. 1962. The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science 5: 393 416.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen
