Ko and Hora's Media for Rhizoctonia
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Purpose
Used for isolation of Rhizoctonia.
Ingredients
| Ingredient | 1 L | 3 L | 8 L | 12 L |
|---|---|---|---|---|
| Part 1 | ||||
| FeSO4 - 7H2O (Ferrous Sulfate) | 0.01 g | 0.03 g | 0.08 g | 0.12 g |
| NaNO2 (Sodium Nitrate) | 0.2 g | 0.6 g | 1.6 g | 2.4 g |
| KCl (Potassium Chloride) | 0.5 g | 1.5 g | 4 g | 6 g |
| MgSO4 - 7H2O | 0.5 g | 1.5 g | 4 g | 6 g |
| K2HPO4 (Potassium Phosphate- Dibasic) | 1 g | 3 g | 8 g | 12 g |
| Part 2 | ||||
| Agar (Morehead) | 12 g | 36 g | 96 g | 144 g |
| Part 3 | ||||
| Gallic acid | 0.4 g | 1.2 g | 3.2 g | 4.8 g |
| 95% EtOH | 2.5 ml | 7.5 ml | 20 ml | 25 ml |
| Part 4 | ||||
| Chloramphenicol | 0.05 g | 0.15 g | 0.4 g | 0.6 g |
| Streptomcin solfate | 0.05 g | 0.15 g | 0.4 g | 0.6 g |
| Alliette | 0.313 g | 0.94 g | 2.50 g | 3.80 g |
Instructions
- Mix the ingredients from part 1 and stir until dissolved.
- Add the agar from part 2 and autoclave.
- Mix together the ingredients in part 3 until dissolved.
- Dissolve Chloramphenicol in the Gallic acid, EtOH mixture.
- Cool to 50°C and add the remaining ingredients from part 4 just before pouring into plates.
Notes
References
Ko. W. & Hora, F.K. 1971. A selective medium for the quantitative determination of Rhizoctonia solani in soil. Phytopathology 61, 707-710.
Contributed by
Contributed by Sherrie Smith, the University of Arkansas; originally created by Ko and Hora.
