Kennedy & Erwin EDTA medium
From Bugwoodwiki
 |
There is a question about this article that needs to be resolved. Please see the discussion tab of this article for further explanation. |
|
Purpose
Used to induce sporangial formation of Phytophthora.
Ingredients
Macrosalts (Actual Concentration)
| Ingredient | |
|---|---|
| KH2PO4 | 0.001 M |
| KNO3 | 0.005 M |
| Ca(NO3)2 • 4H2O | 0.005 M |
| MgSO4•7H2O | 0.002 M |
Microsalts Stock -- 1ml/L
| Ingredient | |
|---|---|
| H3BO4 | 2.86 g |
| MnCl2•H2O | 1.81 g |
| ZnSO4•7H2O | 0.22 g |
Chelated iron stock--1ml/L
| Ingredient | |
|---|---|
| dH2O | to make 1 liter |
| ethylenediamine tetra-acetic acid (EDTA) | 26.1 g |
| KOH | 15 g |
| FeSO4•7H2O | 24.9 g |
| CuSO4•5H2O | 0.08 g |
| (NH4)6MO7O24•4H2O | 0.2 g |
Instructions
- Grow culture on V-8 juice agar.
- Transfer mycelial disks to this medium for 24 hours, then check for sporulation.
Notes
References
- Kennedy, B. W. and D. C. Erwin. Some factors influencing sporangium formation of a Phytophthora species isolated from lucerne in certain salt solutions. 1961. Trans. Brit. Mycol. Soc. 44: 291-297
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Originally created by Robert Wick; contributed by Mary Ann Hansen
