D1 medium
From Bugwoodwiki
Purpose
To differentiate Agrobacterium from Acidovorax, Burkholderia, and Ralstonia
Ingredients
| Ingredient | *1000 mL* | *500 mL* |
|---|---|---|
| Cellobiose | 5.0 g | 2.5 g |
| NH4Cl | 1.0 g | 0.5 g |
| NaH2PO4 | 1.0 g | 0.5 g |
| K2HPO4 | 1.0 g | 0.5 g |
| MgSO4*7H2O | 3.0 g | 1.5 g |
| Malachite green | 3.0 mg | 1.5 mg |
| Agar | 15 g | 7.5 g |
Instructions
- Mix ingredients
- pH to 7.0
- Autoclave
- Cool to 50-55°C
- Pour into petri plates
Notes
References
- Schaad, N.W. 2001. Laboratory Guide for the Identification of Plant Pathogenic Bacteria, 3rd Edition. The American Phytopathological Society, St. Paul, MN. (pp 6, 9)
Contributed by
From the University of Florida Plant Disease Clinic-GNV System Manual: originally created by Anne Vitoreli; contributed by Anne Vitoreli.
