Crystal violet pectin

Purpose

A media to determine the pectolytic activity of bacteria.

Ingredients

Main Recipe

Ingredient	*500 mL*	*200 mL*
NaNO3	1.0 g	0.4 g
0.075% Crystal Violet in 95% EtOH	1 mL	0.4 mL
1N NaOH	4.5 mL	1.8 mL
10% CaCl2*2H2O (aqueous)	1 mL	0.4 mL
Bacto Agar	2.5 g	1.0 g
Na Polypectate	9.0 g	3.6 g

Instructions

1. Combine NaNo₃ with water
2. Heat until near boiling
3. Add Crystal Violet, NaOH, and CaCl₂
4. Pour mixture into a blender
5. While on low speed, add Bacto Agar and Na Polypectate
6. Blend for ~ 10-15s
7. Pour into an autoclavable bottle with adequate headspace
8. spray with anti-foam (if available)
9. Autoclave
10. Pour into petri dishes immediately after removing from autoclave

Notes

• Spot inoculate bacterial colonies on media. If colonies appear concave in the media, bacteria are pectolytic.

Contributed by

From the University of Florida Plant Disease Clinic-GNV mediabook: originally created by Gary Simone; contributed by Anne Vitoreli.