Welcome to the Diagnostician's Cookbook. This site is meant to help share information on techniques used in the diagnosis of plant diseases and disorders.
If you have expertise that you wish to contribute, please contact Joe LaForest and he will make sure you have access to author articles of add to discussions. When authoring, please pay attention to the standard format for diagnosticians recipes and the standard format for diagnostic tests so that we can keep things as consistent as possible. Articles are placed in the proper places using Categories. The NPDN Diagnostic Subcommittee will begin reviewing the recipes and approved procedures will be listed.
Media recipes
Bacterial Non-selective Media
Bacterial Selective Media
- Agrobacterium medium
- CKTM medium
- CMCA (Carboxymethylcellulose agar)
- Corynebacterium medium
- Crystal violet pectate medium (modified)
- Crystal violet pectin
- D1 medium
- Erwinia amylovora selective medium
- Esterase activity (Tween 80 Hydrolysis)
- High sucrose medium
- Hugh-Liefson medium
- Kados D-1 media for Agrobacterium
- Kados D-5 media for Xanthomonas and Agrobacterium
- Ketolactose test medium
- Kings medium B
- Lactose broth
- Miller-Schroth medium
- Miller-Schroth Polypectate medium
- Minimal medium (Pseudomonas)
- Pectolytic enzyme detection media
- SCM (Xanthomonas semi-selective medium)
- Simmons citrate agar
- SX agar
- Tween medium
- Urease production
- Xanthomonas selective medium
- Yeast dextrose carbonate agar
Fruiting Structure Induction Media
- Carnation leaf agar
- Carrot agar
- Carrot bait
- Charcoal water
- Chen-Zentmyer salt solution
- Clarified V8 agar
- Enumeration of Sclerotia of Macrophomina phaseolina from plants
- Enumeration of Sclerotia of Macrophomina phaseolina from soil
- Hempseed agar
- Induction of Phytophthora parasitica zoospores with V-8 broth
- Isolation of sclerotia of Macrophomina phaseolina from soil
- Kennedy and Erwin EDTA medium
- Leonian medium
- Mineral salts medium
- Non-sterile soil extract solution
- Oatmeal agar
- Oatmeal agar (modified)
- Pea broth
- Petri medium
- Potato marmite agar
- Rape seed agar
- Rye medium A (modified with anitbiotics)
- Rye medium A (modified)
- Rye seed medium
- Salt solution for sporangia formation of water molds
- Soil extract
- Stimulate sporulation of Phytophthora cactorum on ginseng
- Super V8 agar (modified)
- V8 agar
- V8 broth
- Vegetable oil nitrate agar
Fungal Non-selective Media
Fungal Selective Media
- 1A Medium
- Alkaline Water Agar
- APDA - Acidified Potato Dextrose Agar
- Armillaria isolation media
- Carrot juice agar
- Chitin agar
- CMR agar
- Cylindrocladium isolation medium
- Cylindrocladium medium
- Czapek solution agar
- Flowers medium
- GN media for Rhizoctonia
- Mefenoxam medium
- Minimal medium (trace elements)
- Modified PPP medium
- Ortho-phenyl phenol agar
- P5ARP-V8 medium
- Paecilomyces buxi medium
- Potassium nitrate agar medium
- RB media for Macrophomina
- Sporulation of Pythium spp.
- Stem canker agar
- Thielaviopsis basicola media
- Thielaviopsis medium
- V8-PARP
Armillaria Media
Aspergillus Media
Cylindrocarpon Media
Fusarium Media
Gaeumannomyces Media
Pyrenochaeta Media
Rhizoctonia Media
Verticillium Media
Inoculum Media
Oomycete Selective Media
- Cornmeal PPP medium
- Cornmeal Water
- P10VP H for Phytophthora megasperma
- PARP
- PARP or PARP H (modified)
- PARP or PARP H (USDA Version)
- PARPH-V8
- Phytophthora medium (P10VP H)
- Phytophthora megasperma selective medium
- PVP or PVP H
- Pythium (PART)
- Pythium medium (P10VP)
- Pythium oligrandrum semi-selective medium
- Pythium selective medium (P5ARP)
- Sand-Corn meal inoculum for pythium
- V8P10H Phytophthora medium
Diagnostic tests
Bacterial Diagnostic Tests
- Acid and/or Gas production from Carbohydrates
- Aerobe-anaerobe test
- Aesculin hydrolysis
- Arbutin hydrolysis
- Arbutin hydrolysis (B-Glucosidase)
- Arbutin Hydrolysis broth
- Arginine dihydrolase medium
- Arginine dihydrolase test
- Asparagine utilization
- Bacterial blight detection from seed
- Bacterial isolation
- Bacterial streaking
- Bacterial streaming
- D2ANX medium
- Decarboxylase medium
- Endospore stain
- Erythromycin test
- Gelatin liquefaction
- Hydrogen sulfide production
- Hypersensitivity Reaction (HR)
- Identification of bacterial genera
- KOH test
- L-asparagine solution
- Lactose broth
- Lecithinase activity
- LOPAT scheme for fluorescent Pseudomonads
- Oxidase test
- Phenylalanine agar
- Phosphatase medium
- Lecithinase activity
- Plate count medium
- Poly-B hydroxyrate accumulation
- Potato soft rot test
- Tests that differentiate pectolytic bacteria
- Tetrazolium medium
- Tobacco hypersensitivity reaction
- Urease production
- Utilization of carbohydrates (standard mineral base)
Conventional PCR
Fungal Diagnostic Tests
- Basidiomycete stain
- Detecting yellow tuft in turfgrasses
- Detection of phytophthora in water supplies
- Fusarium wilt of palms (conventional PCR)
- Fusarium wilt of palms (real-time PCR)
- Lactophenol analine-blue
- Minimal medium (trace elements)
- Seed Washing Test
- Single sporing
- Size-selective sieving technique
General Diagnostic Tests
Nematode Diagnostic Tests
Nested PCR
Oomycete Diagnostic Tests
Sequencing
Fungal culture storage
Bacterial culture storage
Oomycete culture storage
NPDN Diagnostic Tips
- A Novel Approach to Testing with Immunostrips
- A Simple Diagnostic Technique For Identification of Three Wheat Rust Fungi
- A Simple Solution for an Irritating Lab Task
- An Easy Way to Get Pure Cultures from Fungal Leaf Spots
- Carrot Bait for Thielaviopsis basicola
- Checking for Bacterial Streaming
- Clear Tape Techniques for Plant Disease Diagnostics
- Clearing and Staining Roots and Other Plant Tissues
- Diagnostic Lab Floor Plan and Lab Organization
- Diagnostic Uses for the Inverted Microscope
- Double-Stranded RNA Analysis as a Tool for Diagnosing Plant Viruses
- Float Incubation Technique for Plant Disease Diagnostics
- Forceps Technique Useful for Isolating Pathogens
- Forceps Tip
- Improving Isolation Results
- Long term Refrigerator Storage of Didymella bryoniae and Other Fungi on Filter Paper
- Moist-chamber on Water Agar
- Month: Techniques for Frequently Finicky Fungi Fond of Foiling Identification
- Oak Wilt Testing
- Obtaining Pure Bacterial Colonies and an Alternative to the Gram Stain
- Parafilm Dispenser
- Preparation and Storage of Primers and Probes
- Quick Sectioning Technique
- Re-pointing Forceps
- Soybean Dwarf Virus Testing of Soybeans
- Testing for Races of Aphanomyces euteiches in Alfalfa Soils
- Tool to Ease SCN Egg Extractions
- Two Basic Techniques for Enhancing Detection of Sporulating Fungi
- Using Cheap Filters to Soften Lighting Hot Spots for Digital Imaging
- Using Window Screening as a Strainer for Isolation Material
Diagnostic Lab Manuals
- UMass diagnostic manual
- Arkansas Diagnostic Manual
- Virginia Tech Diagnostic Manual
- Kentucky Diagnostic Manual
- Penn State Plant Disease Clinic Manual
Acknowledgments
Thanks to the following individuals and institutions for their contributions to this resource:
- Robert Wick, University of Massachusetts
- Mary Ann Hansen, Virginia Tech
- Sherrie Smith, University of Arkansas
- Tom Creswell, Purdue University
- Gail Ruhl, Purdue University
- Meg Williamson, Clemson University
- Anne Vitoreli, University of Florida
- Paul Vincelli, University of Kentucky
- Paul Bachi, University of Kentucky
|
|
|
|
|
|
 
| |||||
