Arbutin hydrolysis
From Bugwoodwiki
Purpose
Used to identify strains of Pseudomonas syringae. The test detects the presence of the enzyme, β-glucosidase, which is present in some strains of P. syringae and which splits the glucoside, arbutin.
Ingredients
| Ingredient | 500 ml | 250 ml |
|---|---|---|
| Distilled water | 500 ml | 250 ml |
| Arbutin | 2.5 g | 1.25 g |
| Peptone | 5 g | 2.5 g |
| Yeast extract | 1.5 g | 0.75 g |
| Glucose | 0.5 g | 0.25 g |
| Ferric (ammonium) citrate | 0.25 g | 0.125 g |
Instructions
- Dispense in tubes. Autoclave.
- After inoculation dark brown coloration indicates hydrolysis of arbutin. Wait up to 10 days.
Notes
References
Crosse, J. E. & Garrett, C. M. E. 1963. Studies on the bacteriolophagy of Pseudomonas mors-prunorum, P. syringae and related organisms. J. Appl. Bact. 26:159-177.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
