Arbutin Hydrolysis broth
From Bugwoodwiki
Purpose
Used to identify strains of Pseudomonas syringae. The test detects the presence of the enzyme, ß-glucosidase, which is present in some strains of P. syringae and which splits the glucoside, arbutin.
Ingredients
| Ingredient | 500 ml | 250 ml |
|---|---|---|
| Arbutin | 2.5 g | 1.25 g |
| Peptone | 5 g | 2.5 g |
| Yeast extract | 1.5 g | 0.75 g |
| Glucose | 0.5 g | 0.25 g |
| Ferric (ammonium) citrate | 0.25 g | 0.125 g |
Instructions
- Dispense ingredients into tubes and autoclave.
- After inoculation of tubes with isolates, dark brown coloration indicates hydrolysis of arbutin. Wait up to 10 days.
Notes
References
- Crosse, J. E. and C. M. E. Garrett. 1963. Studies on the bacteriolophagy of Pseudomonas mors prunorum, P. syringae and related organisms. J. Appl. Bact. 26:159 177.
Contributed by
From the Virginia Polytechnic Institute and State University Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen
