Aesculin hydrolysis
From Bugwoodwiki
Purpose
Aesculin utilization is diagnostic for Xanthomonas campestris, X. alnilineans, and X. axonopodis
Ingredients
| Ingredient | 500 ml | 250 ml |
|---|---|---|
| Distilled water | 500 ml | 250 ml |
| NH4H2PO4 | 0.25 g | 0.125 g |
| K2HPO4 | 0.25 g | 0.125 g |
| MgSO4 • 7H2O | 0.1 g | 0.05 g |
| NaC1 | 2.5 g | 1.25 g |
| Yeast Extract | 2.5 g | 1.25 g |
| Ferric ammonium citrate | 0.25 g | 0.125 g |
| Aesculin | 0.5 g | 0.25 g |
Instructions
- Development of a dark brown color in aesculin broth after shaker incubation for up to 28 days is regarded as an indication of aesculin utilization
- Complete hydrolysis can be assumed when no fluorescence is seen under UV light.
Notes
References
- Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory guide for the identification of plant pathogenic bacteria, 3rd edition. The American Phytopathological Society, St. Paul, MN. 373pp.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
