ATP extraction
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Purpose
general soil biological activity
Ingredients
- 100 g soil
- 20 ml extract
- 0.1 M Tricine buffer pH 11.2
| # of samples | 4 | 8 | 12 | 16 |
|---|---|---|---|---|
| Lubrol solution | 34 | 68 | 102 | 136 |
| Phosphoric acid | 20 | 40 | 60 | 80 |
| Urea | 20 | 40 | 60 | 80 |
| DMSO | 20 | 40 | 60 | 80 |
| Adenosine | 4 | 8 | 12 | 16 |
| EDTA | 2 | 4 | 6 | 8 |
Instructions
- Blend soil and extract for 20 seconds, 3 times.
- Centrifuge at 3000 G in Sorval SS-1 at room temp. for 20 minutes.
- Dilute 1:10 with Tricine buffer.
- Check if pH is between 7 and 8
- Place samples in freezer
- Lubrol dissolve 0.5 g in 34 ml sterile H2O by warming hold at 35°C
Notes
- Add EDTA just before use
References
Contributed by
Contributed by Sherrie Smith, the University of Arkansas
