Simmon's citrate agar
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Contents |
Purpose
This medium is used to aid in the differentiation of various genera in the Enterobacteriaceae. Bacteria that can utilize citrate as the sole carbon source and inorganic ammonium salt as the sole source of nitrogen grow on this medium.
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Distilled water | 1000 ml | 500 ml |
| MgSO4 • 7H2O | 0.2 g | 0.1 g |
| NH4H2PO4 | 1 g | 0.5 g |
| K2HPO4 | 1 g | 0.5 g |
| Sodium citrate | 2 g | 1 g |
| NaCl | 5 g | 2.5 g |
| Bacto agar | 15 g | 7.5 g |
| Bacto bromothymol blue | 0.8 g | 0.4 g |
Instructions
- Medium is usually prepared as agar slants, inoculated by lightly streaking the slant.
- Incubate up to 4 days at 37 °C.
Notes
- Bacteria that can utilize citrate as the sole carbon source and inorganic ammonium salt as the sole source of nitrogen grow on this medium.
Expected results
- A positive reaction is indicated by growth with an intense blue color on the slant (alkalinity). A negative reaction is no growth with no color change; the medium remains dark green.
References
- Difco Manual, 10th Ed. 1984. Difco Laboratories, Detroit, MI. 1155 pp. (p. 864). MacFadden, J. F. 1985. Media for Isolation-Cultivation-Identification
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
