SX agar
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Contents |
Purpose
Semiselective medium for isolation of starch-positive pathovars of Xanthomonas campestris (X. c. pvs. campestris, begoniae, fragaria, and corylina) from soil or plant tissues. Also useful for direct plating of contaminated seeds.
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Distilled water | 1000 ml | 500 ml |
| Soluble potato starch | 10 g | 5 g |
| Beef extract | 1 g | 0.5 g |
| Ammonium chloride | 5 g | 2.5 g |
| K2 HPO41 | 2 g | 1 g |
| Agar | 15 g | 7.5 g |
| Methyl violet 2B (1% solution in 20% ethanol) | 1 ml | 0.5 ml |
| Methyl green (1% solution) | 2 ml | 1 ml |
| Cycloheximide (optional)* | 0.25 g | 0.125 g |
Instructions
Notes
- The medium should be violet in color when melted. Fahy (Plant Bacterial Diseases) incorrectly lists the wrong potassium salt, which results in a blue slush medium. It is also incorrect in the original paper1. The above, K2HPO4 is correct.
- Cycloheximide can be added to inhibit fungi from growing on the medium.
References
- Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory guide for the identification of plant pathogenic bacteria, 3rd edition. The American Phytopathological Society, St. Paul, MN. 373pp.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
