Oatmeal agar (modified)
From BugwoodWiki
Contents |
Purpose
The following modification of oatmeal agar is used to induce sporulation of many isolates of Botryosphaeria dothidea and other Botryosphaeria species. Most isolates sporulate in about 14 days when kept under continuous fluorescent illumination (8-10 inches below a fixture containing two 34 Watt tubes). Scoring or scraping also speeds sporulation of some intractable isolates.
Ingredients
| Ingredient | |
|---|---|
| dH2O | 1 L |
| Quick-cooking oats (ground {dry} in a blender) | 50 g |
| Agar | 15 g |
| Silicone defoarmer spray* | 1 ml |
Instructions
- Dissolve agar in boiling water and thoroughly mix in oatmeal.
- Add defoamer immediately before autoclaving; if bottling in aliquots, allow more headroom in the bottles than for other types of media.
- This medium must be poured hot to keep the oatmeal well suspended.
Notes
- Principal ingredient is dimethylpolysiloxane; available from Kalo Agricultural Chemicals, Overlook Park, Kansas, (913) 491-9125.*
References
- Tom Creswell, North Carolina State University.
Contributed by
From the Virginia Tech Mediabook; Originally created by Robert Wick; contributed by Mary Ann Hansen
