Oatmeal agar

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Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

Oatmeal Agar is used for cultivating fungi, particularly for macrospore formation.

Ingredients

Ingredient	1 L	500 ml
Distilled water	1000 ml	500 ml
Rolled Oats	30 g	15 g
Agar	15 g	7.5 g

Instructions

1. Cook oatmeal in water for 15 – 30 minutes.
2. Filter through three or four layers of cheesecloth and bring filtrate back to volume with water.
3. Add agar. Autoclave to sterilize.

Notes

• To induce abundant sporangial formation in P. parasitica var. nicotianae:

1. Transfer aerial mycelium from 15- to 20-day old cultures to a Petri dish containing enough 0.01 M KNO₃ to wet the mycelium but not submerge it.
2. Incubate 2-10 days at room temperature.

References

Modified from Gooding, G. V. and G. B. Lucas. 1959. Factors influencing sporangial formation and zoospore activity in Phytophthora parasitica var. nicotianae. Phytopath. 49:277-281.

Contributed by

From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick