Minimal medium (Pseudomonas)

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Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

For culturing pseudomonas.

Ingredients

Ingredient	1 L	500 ml
Distilled water	985 ml	492.5 ml
Glycerol	15 ml	7.5 ml
L-Glutamine	5 g	2.5 g
K2HPO4	1.5 g	0.75 g
MgSO4	0.2 g	0.1 g
Agar	15 g	7.5 g

Instructions

1. Adjust pH to 7.0
2. Autoclave and cool.

Notes

References

Contributed by

From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick