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Purpose
Ingredients
| Ingredient
| 1 L
| 500 ml
|
| Distilled water
| 1000 ml
| 500 ml
|
| Sucrose
| 30 g
| 15 g
|
| KH2PO4
| 1 g
| 0.5 g
|
| MgSO4 • 7H2O
| 0.5 g
| 0.25 g
|
| KCl
| 0.5 g
| 0.25 g
|
| FeSO4 • 7H2O
| 0.01 g
| 0.005 g
|
| Agar
| 20 g
| 10 g
|
| Trace element solution*
| 0.2 ml
| 0.1 ml
|
| L-Asparagine
| 1.6 g
| 0.8 g
|
| NaNO3
| 2 g
| 1 g
|
| KClO3
| 15 g
| 7.5 g
|
Trace element solution*
| Ingredient
| 100 ml
|
| Distilled water
| 95 ml
|
| Citric acid
| 5 g
|
| ZnSO4 • 7H2O
| 5 g
|
| FeSO4 • 7H2O
| 4.75 g
|
| Fe(NH4)2(SO4)2 • 6H2O
| 1 g
|
| CuSO4 • 5H2O
| 250 mg
|
| MnSO4 • H2O
| 50 mg
|
| H3BO3
| 50 mg
|
| Na2MoO4 • 2H2O
| 50 mg
|
Instructions
Notes
References
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick