Leonian medium

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Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

Used for stimulating pycnidium formation in Sphaeropsidales.

Ingredients

Ingredient
dH2O	1 L
Dihydrogen potassium phosphate	1.25 g
Magnesium sulfate	0.625 g
Peptone	0.625 g
Maltose	6.25 g
Malt extract	6.25 g

Instructions

1. Heat solution in a double boiler or in Arnold’s sterilizer for 15 min.
2. Filter.
3. Autoclave at 10 pounds pressure for 15 min.
4. Add 1.5-2% agar for solid media. Agar should be melted in a separate lot of water before it is added to the nutrient portion.

Notes

References

• Leonian, L. H. 1924. A study of factors promoting pycnidium formation in some Sphaeropsidales. Amer. Jour. Bot. 11:19-50.

Contributed by

From the Virginia Tech Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen