Komada medium

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Contents

Purpose

Selective for Fusarium and is reported to distinguish F. oxysporum, F. solani, F. moniliforme, and F. roseum on the basis of colony color. Galactose is preferentially used by Fusarium species.

Ingredients

Ingredient 1 L 500 mL
Part 1
distilled water 1 L 500 ml
Na2B4O7 • 10 H2O 1 g 0.5 g
K2HPO4 1 g 0.5 g
KCl 0.5 g 0.25 g
MgSO4 • 7 H2O 0.5 g 0.25 g
Fe-Na-EDTA 0.01 g 0.005 g
D-galactose 20 g 10 g
L-asparagine 2 g 1 g
agar 15 g 7.5 g
PCNB (Terraclor 75 % WP)(pentacloronitrobenzene) 1.0 g 0.5 g
Part 2
oxgall (Bile Bovine) 0.5 g 0.25 g
streptomycin sulfate 0.3 g 0.15 g

Instructions

  1. Mix ingredients in Part 1
  2. Heat to boiling to melt agar. Do not autoclave.
  3. Cool to 50 55°C
  4. Add Part 2
  5. Acidify the medium to 3.8 4.0 pH with 10% phosphoric acid. (It will take about 8 ml).

Notes

  • Chloramphenicol can be substituted for streptomycin sulfate at a rate of 0.25 g/liter.

References

  • Komada, H. 1976. A new selective medium for isolating Fusarium from natural soil. Proceedings of the American Phytopathological Society 3:221

Contributed by

From the Virginia Tech Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen

Images

5364061
Photo by Howard F. Schwartz, Colorado State University, Bugwood.org

View in Bugwood Image Database
5364062
Photo by Howard F. Schwartz, Colorado State University, Bugwood.org

View in Bugwood Image Database
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