Selective for Fusarium and is reported to distinguish F. oxysporum, F. solani, F. moniliforme, and F. roseum on the basis of colony color. Galactose is preferentially used by Fusarium species.
|Ingredient||1 L||500 mL|
|distilled water||1 L||500 ml|
|Na2B4O7 • 10 H2O||1 g||0.5 g|
|K2HPO4||1 g||0.5 g|
|KCl||0.5 g||0.25 g|
|MgSO4 • 7 H2O||0.5 g||0.25 g|
|Fe-Na-EDTA||0.01 g||0.005 g|
|D-galactose||20 g||10 g|
|L-asparagine||2 g||1 g|
|agar||15 g||7.5 g|
|PCNB (Terraclor 75 % WP)(pentacloronitrobenzene)||1.0 g||0.5 g|
|oxgall (Bile Bovine)||0.5 g||0.25 g|
|streptomycin sulfate||0.3 g||0.15 g|
- Mix ingredients in Part 1
- Heat to boiling to melt agar. Do not autoclave.
- Cool to 50 55°C
- Add Part 2
- Acidify the medium to 3.8 4.0 pH with 10% phosphoric acid. (It will take about 8 ml).
- Chloramphenicol can be substituted for streptomycin sulfate at a rate of 0.25 g/liter.
- Komada, H. 1976. A new selective medium for isolating Fusarium from natural soil. Proceedings of the American Phytopathological Society 3:221
From the Virginia Tech Mediabook; Orignially created by Robert Wick; contributed by Mary Ann Hansen