Esterase activity (Tween 80 Hydrolysis)

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Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

Used to detect the ability of bacteria to hydrolyze the lipid, Tween 80.

Ingredients

Ingredient	1 L	500 ml
Distilled water	1000 ml	500 ml
Peptone	10 g	5 g
NaCl	5 g	2.5 g
CaCl2 • 2H20	0.1 g	0.05 g
Agar	15 g	7.5 g

Instructions

1. Adjust pH to 7.4.
2. Autoclave and add Tween 80 (autoclaved) at 10 ml/liter rate.
3. Pour into Petri plates.
4. Spot or streak bacterial isolates. Esterase will hydrolyze the tween and subsequent precipitation with the calcium chloride will appear as a turbid zone around the colonies.

Notes

References

• Lelliott, R. A. and D. E. Stead. 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Blackwell Sci. Pub. 216 pp. ( pp. 195-196).

Contributed by

From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick