Diagnostician's Cookbook
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Welcome to the Diagnostician's Cookbook. This site is meant to help share information on techniques used in the diagnosis of plant diseases and disorders.
If you have expertise that you wish to contribute, please contact Joe LaForest and he will make sure you have access to author articles of add to discussions. When authoring, please pay attention to the standard format for diagnosticians recipes and the standard format for diagnostic tests so that we can keep things as consistent as possible. Articles are placed in the proper places using Categories.
Contents |
Media recipes
- 0.02M Phosphate buffer
- 1/10 TSA agar
- ATP extraction
- CMR agar
- Chitin agar
- Dilute water agar for soil dilution
- Luria broth
- MMC medium
- Macrophomina Inoculum
- Martin's rose agar
- Microwave pasteurization of soil
- NBY-Mn agar
- Phosphate buffer
- Richard's V8 + sucrose
- Simmon's citrate agar
- Soil Microbial Population
- Soil dilution
- Soil-potato inoculum
- Stem Canker agar
- WArad - for isolation from roots
- YPPS
- Yeast nutrient agar
Bacterial Non-selective Media
Bacterial Selective Media
- Agrobacterium medium
- CKTM medium
- CMCA (Carboxymethylcellulose agar)
- Corynebatcerium medium
- Crystal violet pectate medium (modified)
- Crystal violet pectin
- D1M agar
- Erwinia amylovora selective medium
- Esterase activity (Tween 80 Hydrolysis)
- High sucrose medium
- Hugh-Liefson medium
- Kado's D-1 media for Agrobacterium
- Kado's D-5 media for Xanthomonas and Agrobacterium
- Ketolactose test medium
- Kings medium B
- Lactose broth
- Miller-Schroth medium
- Minimal medium (Pseudomonas)
- Pectolytic enzyme detection media
- SCM (Xanthomonas semi-selective medium)
- SX agar
- Tween medium
- Urease production
- Xanthomonas selective medium
- Yeast extract dextrose
Fruiting Structure Induction Media
- Carnation leaf agar
- Carrot agar
- Carrot bait
- Charcoal water
- Chen-Zentmyer salt solution
- Clarified V8 agar
- Enumeration of Sclerotia of Macrophomina phaseolina from plants
- Enumeration of Sclerotia of Macrophomina phaseolina from soil
- Hempseed agar
- Induction of Phytophthora parasitica zoospores with V-8 broth
- Isolation of sclerotia of Macrophomina phaseolina from soil
- Kennedy & Erwin EDTA medium
- Leonian medium
- Non-sterile soil extract solution (1.5%)
- Oatmeal agar
- Oatmeal agar (modified)
- Pea broth
- Petri medium
- Potato marmite agar
- Rape seed agar
- Rye medium A (modified with anitbiotics)
- Rye medium A (modified)
- Rye seed medium
- Salt solution for sporangia formation of water molds
- Soil extract
- Stimulate sporulation of Phytophthora cactorum on ginseng
- Super V8 agar (modified)
- V8 agar
- V8 broth
- Vegetable oil nitrate agar
Fungal Non-selective Media
- Acid potato dextrose agar
- Acid potato dextrose agar (half strength)
- Actidione malt agar
- Lima bean agar
- Malt extract agar
- Ohio medium
- PDArad - for isolation from roots
- Potato dextrose agar
- Potato dextrose agar (quarter strength)
- Synthetically nutrient poor agar
- Water agar
Fungal Selective Media
- Armillaria isolation media
- Carrot juice agar
- Cylindrocladium isolation medium
- Cylindrocladium medium
- Czapek solution agar*
- Flower's medium
- GN + media for Rhizoctonia
- Mefenoxam medium
- Minimal medium (trace elements)
- Ortho-phenyl phenol agar
- P5ARP-V8 medium
- Paecilomyces buxi medium
- Potassium nitrate agar medium
- RB media for Macrophomina
- Sporulation of Pythium spp.
- Thielaviopsis basicola media
- Thielaviopsis medium
Armillaria Media
Aspergillus Media
Cylindrocarpon Media
Fusarium Media
- Carnation leaf agar
- Carrot agar
- Fusarium selective medium
- Komada medium
- Komada-Fusarium medium
- Malachite green agar
- Minimal medium (Fusarium)
- Nash & Snyder Fusarium medium (modified)
- PCNB medium
- Papavizas medium
- Spezieller Nährstoffarmer agar
Gaeumannomyces Media
Pyrenochaeta Media
Rhizoctonia Media
- Antibiotic water agar
- Flowers media
- Ko and Hora's Media for Rhizoctonia
- TAB for Rhizoctonia
- Thiophanate methyl agar
Verticillium Media
- Alcohol agar
- EtOH agar
- Verticillium dahliae microsclerotia production
- Verticillium medium
- Verticillium population medium
Oomycete Selective Media
- Cornmeal PPP medium
- Cornmeal Water
- P10VP+H for Phytophthora megasperma
- PARP
- PARP or PARP + H (modified)
- PARP or PARP+H (USDA Version)
- PVP or PVP+H
- Phytophthora medium (P10VP+H)
- Phytophthora megasperma selective medium
- Pythium (PART)
- Pythium medium (P10VP)
- Pythium oligrandrum semi-selective medium
- Pythium selective medium (P5ARP)
- Sand-Corn meal inoculum for pythium
- V8P10H Phytophthora medium
Diagnostic tests
Bacterial Diagnostic Tests
- Acid and/or Gas production from Carbohydrates
- Aerobe-anaerobe test
- Aesculin hydrolysis
- Arbutin hydrolysis
- Arbutin hydrolysis (B-Glucosidase)
- Arginine dihydrolase medium
- Arginine dihydrolase test
- Asparagine utilization
- Bacterial isolation
- Bacterial streaking
- Bacterial streaming
- D2ANX medium
- Decarboxylase medium
- Endospore stain
- Erythromycin test
- Gelatin liquefaction
- Hydrogen sulfide production
- Hypersensitivity Reaction (HR)
- Identification of bacterial genera
- KOH test
- LOPAT scheme for fluorescent Pseudomonads
- Lactose broth
- Lecithinase activity
- Oxidase test
- Phenylalanine agar
- Phosphatase medium
- Phospholipase activity
- Plate count medium
- Poly-B hydroxyrate accumulation
- Potato soft rot test
- Pseudomonas syringae pv. syringae (Real-time PCR)
- Tests that differentiate pectolytic bacteria
- Tetrazolium medium
- Tobacco hypersensitivity reaction
- Urease production
- Utilization of carbohydrates (standard mineral base)
Conventional PCR
- Detection of BegomoViruses Using Degenerate Primers
- Detection of Brettanomyces in wine
- Detection of Gaeumannomyces graminis var avenae
- Extraction of Total DNA from Bermudagrass
- Fusarium wilt of palms (conventional PCR)
- Ophiosphaerella herpotricha
- Ophiosphaerella korrae
- Ophiosphaerella namari
- PCR Detection of Tomato Yellow Leaf Curl Virus
- Palm phytoplasmas
- Pseudomonas syringae pv. syringae
- Pseudomonas syringae pv. tomato
- Ralstonia solanacearum
- Serratia marcescens
- Soybean Cyst Nematode Detection PCR
- Xanthomonads on Tomato and Pepper
- Xylella ITS Taqman Assay
Fungal Diagnostic Tests
- Basidiomycete stain
- Detecting yellow tuft in turfgrasses
- Detection of phytophthora in water supplies
- Fusarium wilt of palms (conventional PCR)
- Fusarium wilt of palms (real-time PCR)
- Lactophenol analine-blue
- Minimal medium (trace elements)
- Seed Washing Test
- Single sporing
- Size-selective sieving technique
General Diagnostic Tests
- Agdia pathoscreen indirect ELISA
- Agidia Phytophthora Pathoscreen DAS ELISA
- Autoclaving culture media
- ELISA enzyme conjugate preparation (peroxidase)
- Enzyme conjugate preparation (alkaline phosphatatse)
- Palm phytoplasmas
Nested PCR
Oomycete Diagnostic Tests
Real-time PCR
- Fusarium wilt of palms (real-time PCR)
- Pseudomonas syringae pv. syringae (Real-time PCR)
- SmartCycler Additive Reagent
Sequencing
- Distinguishing fungi by ITS sequencing
- Identification Species of powedery mildew pathogens
- Identification of Phytophthora Spp. (direct sequencing)
Viral Diagnostic Tests
- Mechanical Inoculation of plants with tomato spotted wilt virus
- Preparation of materials for substrate-chromophore
- Protocol for tissue blot detection of viral antigens
- Tissue blot technique for detection of viruses
- Virus inclusion stains
- Virus inclusions
Other related topics
Fungal culture storage
Bacterial culture storage
Oomycete culture storage
NPDN Diagnostic Tips
- A Novel Approach to Testing with Immunostrips
- A Simple Diagnostic Technique For Identification of Three Wheat Rust Fungi
- A Simple Solution for an Irritating Lab Task
- An Easy Way to Get Pure Cultures from Fungal Leaf Spots
- Carrot Bait for Thielaviopsis basicola
- Checking for Bacterial Streaming
- Clear Tape Techniques for Plant Disease Diagnostics
- Clearing and Staining Roots and Other Plant Tissues
- Diagnostic Lab Floor Plan and Lab Organization
- Diagnostic Uses for the Inverted Microscope
- Double-Stranded RNA Analysis as a Tool for Diagnosing Plant Viruses
- Float Incubation Technique for Plant Disease Diagnostics
- Forceps Technique Useful for Isolating Pathogens
- Forceps Tip
- Improving Isolation Results
- Long term Refrigerator Storage of Didymella bryoniae and Other Fungi on Filter Paper
- Long-term Freezer Storage of Didymella bryoniae on Filter Paper
- Moist-chamber on Water Agar
- Month: Techniques for Frequently Finicky Fungi Fond of Foiling Identification
- Oak Wilt Testing
- Obtaining Pure Bacterial Colonies and an Alternative to the Gram Stain
- Parafilm Dispenser
- Preparation and Storage of Primers and Probes
- Quick Sectioning Technique
- Re-pointing Forceps
- Soybean Dwarf Virus Testing of Soybeans
- Testing for Races of Aphanomyces euteiches in Alfalfa Soils
- Tool to Ease SCN Egg Extractions
- Two Basic Techniques for Enhancing Detection of Sporulating Fungi
- Using Cheap Filters to Soften Lighting Hot Spots for Digital Imaging
- Using Window Screening as a Strainer for Isolation Material
Diagnostic Lab Manuals
Acknowledgments
Thanks to the following individuals and institutions for their contributions to this resource:
- Robert Wick, University of Massachusetts
- Mary Ann Hansen, Virginia Tech
- Sherrie Smith, University of Arkansas
- Tom Creswell, Purdue University
- Gail Ruhl, Purdue University
- Meg Williamson, Clemson University
- Anne Vitoreli, University of Florida
- Paul Vincelli, University of Kentucky
- Paul Bachi, University of Kentucky
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