D1M agar

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Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

To differentiate Agrobacterium from Acidovorax, Burkholderia, and Ralstonia

Ingredients

Ingredient	*1000 mL*	*500 mL*
Cellobiose	5.0 g	2.5 g
NH4Cl	1.0 g	0.5 g
NaH2PO4	1.0 g	0.5 g
K2HPO4	1.0 g	0.5 g
Malachite green	3.0 mg	1.5 mg
Agar	15 g	7.5 g

Instructions

1. Mix ingredients
2. pH to 7.0
3. Autoclave
4. Cool to 50-55°C
5. Pour into petri plates

Notes

References

• Schaad, N.W. 2001. Laboratory Guide for the Identification of Plant Pathogenic Bacteria, 3rd Edition. The American Phytopathological Society, St. Paul, MN. (pp 6, 9)

Contributed by

From the University of Florida Plant Disease Clinic-GNV System Manual: originally created by Anne Vitoreli; contributed by Anne Vitoreli.