Chen-Zentmyer salt solution
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Contents |
Purpose
Salt solution is necessary to induce sporangia formation and zoosporogenesis of several species of Phytophthora.
Ingredients
| Ingredient | 2 L | 1 L |
|---|---|---|
| Part 1 | ||
| Distilled Water | 2000 ml | 1000 ml |
| Ca(NO3)2-4H20 | 4.72 g | 2.36 g |
| MgSO4-7H20 | 1.972 g | 0.986 g |
| KNO3 | 1.020 g | 0.510 g |
| Part 2 | ||
| Sequestrene 330 (10% Fe, 0.05 mg/l) | 2 ml | 1 ml |
| or | ||
| Sequestrene 138 (6% Fe, 0.084 mg/l) | 2 ml | 1 ml |
Instructions
- Add ingredient from part 2 to media.
- Adjust pH to 7.0 with 2.4 drops 20 N NaOH. Autoclave.
- Filter-sterilize into autoclaved solution. This results in a total of 5x10-3 mg Fe/l.
Notes
- Alternatively, iron may be added after autoclaving as 0.02 mg FeSO4 • 7H2O/l by filter sterilization through a 0.22μ filter. Schmitthenner version uses Sequestrene. The reference cited below uses chelated iron prepared as follows:
EDTA........................................................................................1.3 g
KOH........................................................................................0.75 g
FeSO4 • 7H2O ........................................................................0.49 g
Deionized water ....................................................................100.0 ml
- Small agar blocks of culture (about 3 mm) are irrigated several times every two to four hours.
References
- Mycologia 62:397-402. Schmitthenner, personal communication.
- Chen, D. W. and G. A. Zentmyer. 1970. Production of sporangia by Phytophthora cinnamomi in axenic culture. Mycologia 62: 397-402.
- Schmitthenner, A. F. , personal communication.
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
