Carnation leaf agar

From BugwoodWiki

Contents 1 Purpose 2 Ingredients 3 Instructions 4 Notes 5 References 6 Contributed by

Purpose

Sporulation of Fusarium spp.

Ingredients

Ingredient	*1L*	*500mL*
Granulated Agar	8.4 g	4.2 g

Instructions

1. Mix granulated agar with DI water
2. Autoclave and cool to 50-55°C
3. Pour into petri plates
4. Sprinkle on 3-5 irradiated carnation leaf pieces
5. Let set for at least 3 days to determine if there is any contamination

Notes

• Irradiated carnation leaves can be ordered through Penn State's Fusarium Lab
• Can use irradiated banana leaf pieces if carnation leaves are not available

References

• Leslie, J.F. and Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Ames, IA p. 6

Contributed by

From the University of Florida Plant Disease Clinic-GNV mediabook: originally created by Gary Simone; contributed by Anne Vitoreli.