CMCA (Carboxymethylcellulose agar)
From BugwoodWiki
Contents |
Purpose
Used to test bacteria (or fungi) for cellulolytic activity.
Ingredients
| Ingredient | 1 L | 500 ml |
|---|---|---|
| Part 1 | ||
| Distilled water | 1000 ml | 500 ml |
| NH4H2PO4 | 1 g | 0.5 g |
| KCl | 0.2 g | 0.1 g |
| MgSO4 • 7H2O | 1 g | 0.5 g |
| Yeast extract | 1 g | 0.5 g |
| Part 2 | ||
| Carboxymethyl cellulose | 26 g | 13 g |
| Agar | 3 g | 1.5 g |
Instructions
- Mix ingredients from part 1 and add ingredients from part 2 while stirring.
- Autoclave for 20 minutes
Notes
References
Contributed by
From the Culture Media for Plant Pathogenic Fungi and Bacteria, University of Massachusetts: Contributed by Robert L. Wick
