Agrobacterium medium
From BugwoodWiki
Contents |
Purpose
For isolation of Agrobacterium tumefaciens-A. radiobacter group from soil.
Ingredients
| Ingredient | ||
|---|---|---|
| Part 1 | ||
| H2O | 1 L | |
| Mannitol | 10 g | |
| Sodium nitrate | 4 g | |
| Magnesium chloride | 2 g | |
| Calcium propionate | 1.2 g | |
| Magnesium phosphate | 0.2 g | |
| Magnesium sulfate | 0.1 g | |
| Sodium bicarbonate | 0.075 g | |
| Magnesium carbonate | 0.075 g | |
| Agar | 20 g | |
| Part 2 | ||
| Berberine | 275 mg | |
| Sodium selinite | 100 mg | |
| Penicillin G (1625 units/mg) | 60 mg | |
| Streptomycin sulfate (78% strep base) | 30 mg | |
| Cycloheximide (80-100% a.i.) | 250 mg | |
| tyrothricin (pure) | 1 mg | |
| bacitracin (65 unit/mg) | 100 mg | |
Instructions
- Autoclave ingredients from part 1 and cool to 50-55°C.
- Add ingredients from part 2
- Adjust pH to 7.1 with 1.0 N HCl.
Notes
References
- Clark, A. G. 1969. A selective medium for the isolation of Agrobacterium species. Journal of Applied Bacteriology 32: 348-351.
- Schroth, M. N., J. P. Thompson and D. C. Hildebrand. 1965 Isolation of Agrobacterium tumefaciens-A. radiobacter group from the soil. Phytopathology 55: 645-647.
- Kado, C. I. and M. G. Heskett. 1970. Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia. Phytopathology 60: 969-976.
Contributed by
From the Virginia Tech Mediabook; Originally created by Robert Wick; contributed by Mary Ann Hansen
